[Optimization of expression condition of SARS-CoV PUPs genes in E. coli].
Identifieur interne : 003534 ( Main/Exploration ); précédent : 003533; suivant : 003535[Optimization of expression condition of SARS-CoV PUPs genes in E. coli].
Auteurs : Jian-Qiang Kong [République populaire de Chine] ; Wei Wang ; Guan-Hua Du ; Ping Zhu ; Ke-Di ChengSource :
- Yao xue xue bao = Acta pharmaceutica Sinica [ 0513-4870 ] ; 2007.
Descripteurs français
- KwdFr :
- Cadres ouverts de lecture, Escherichia coli (génétique), Escherichia coli (métabolisme), Gènes viraux, Génome viral, Protéines de la matrice virale (génétique), Protéines de la matrice virale (métabolisme), Protéines recombinantes (génétique), Protéines recombinantes (métabolisme), Protéines virales (génétique), Protéines virales (métabolisme), Régulation de l'expression des gènes viraux, Temps, Température, Vecteurs génétiques, Virus du SRAS (génétique).
- MESH :
- génétique : Escherichia coli, Protéines de la matrice virale, Protéines recombinantes, Protéines virales, Virus du SRAS.
- métabolisme : Escherichia coli, Protéines de la matrice virale, Protéines recombinantes, Protéines virales.
- Cadres ouverts de lecture, Gènes viraux, Génome viral, Régulation de l'expression des gènes viraux, Temps, Température, Vecteurs génétiques.
English descriptors
- KwdEn :
- Escherichia coli (genetics), Escherichia coli (metabolism), Gene Expression Regulation, Viral, Genes, Viral, Genetic Vectors, Genome, Viral, Open Reading Frames, Recombinant Proteins (genetics), Recombinant Proteins (metabolism), SARS Virus (genetics), Temperature, Time, Viral Matrix Proteins (genetics), Viral Matrix Proteins (metabolism), Viral Proteins (genetics), Viral Proteins (metabolism).
- MESH :
- chemical , genetics : Recombinant Proteins, Viral Matrix Proteins, Viral Proteins.
- genetics : Escherichia coli, SARS Virus.
- metabolism : Escherichia coli, Recombinant Proteins, Viral Matrix Proteins, Viral Proteins.
- Gene Expression Regulation, Viral, Genes, Viral, Genetic Vectors, Genome, Viral, Open Reading Frames, Temperature, Time.
Abstract
According to previous studies of SARS-CoV (Severe acute respiratory syndrome coronavirus), a variety of novel accessory genes have been identified in SARS-CoV genome, which were interspersed the structural genes of SARS-CoV and considered to be unique to the SARS-CoV genome. The predicted unknown proteins (PUPs) encoded by the accessory genes might play important roles in the SARS-CoV infection. Three of those genes, called X4, X5 and ORF10, were synthesized and introduced into E. coli to induce expression. SDS-PAGE and Western blotting revealed that the three genes have been expressed in E. coli. The induction of SARS PUPs genes expression in different temperatures, induction times, IPTG concentrations and A values of E. coli cells were performed. The optimal induction condition of SARS-CoV PUPs genes was characterized according to the orthorgonal analysis. The ratio of recombinant proteins of PUPs to total proteins is as follows: X4, 20%; X5, 27.8%; ORF10, 68.5% under the optimum conditions.
PubMed: 18050746
Affiliations:
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Du</name></author><author><name sortKey="Zhu, Ping" sort="Zhu, Ping" uniqKey="Zhu P" first="Ping" last="Zhu">Ping Zhu</name></author><author><name sortKey="Cheng, Ke Di" sort="Cheng, Ke Di" uniqKey="Cheng K" first="Ke-Di" last="Cheng">Ke-Di Cheng</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:18050746</idno><idno type="pmid">18050746</idno><idno type="wicri:Area/PubMed/Corpus">001C63</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001C63</idno><idno type="wicri:Area/PubMed/Curation">001C63</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001C63</idno><idno type="wicri:Area/PubMed/Checkpoint">001C18</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001C18</idno><idno type="wicri:Area/Ncbi/Merge">001B30</idno><idno type="wicri:Area/Ncbi/Curation">001B30</idno><idno 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type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name></author><author><name sortKey="Du, Guan Hua" sort="Du, Guan Hua" uniqKey="Du G" first="Guan-Hua" last="Du">Guan-Hua Du</name></author><author><name sortKey="Zhu, Ping" sort="Zhu, Ping" uniqKey="Zhu P" first="Ping" last="Zhu">Ping Zhu</name></author><author><name sortKey="Cheng, Ke Di" sort="Cheng, Ke Di" uniqKey="Cheng K" first="Ke-Di" last="Cheng">Ke-Di Cheng</name></author></analytic><series><title level="j">Yao xue xue bao = Acta pharmaceutica Sinica</title><idno type="ISSN">0513-4870</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Escherichia coli (genetics)</term><term>Escherichia coli (metabolism)</term><term>Gene Expression Regulation, Viral</term><term>Genes, Viral</term><term>Genetic Vectors</term><term>Genome, Viral</term><term>Open Reading Frames</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (metabolism)</term><term>SARS Virus (genetics)</term><term>Temperature</term><term>Time</term><term>Viral Matrix Proteins (genetics)</term><term>Viral Matrix Proteins (metabolism)</term><term>Viral Proteins (genetics)</term><term>Viral Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cadres ouverts de lecture</term><term>Escherichia coli (génétique)</term><term>Escherichia coli (métabolisme)</term><term>Gènes viraux</term><term>Génome viral</term><term>Protéines de la matrice virale (génétique)</term><term>Protéines de la matrice virale (métabolisme)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (métabolisme)</term><term>Protéines virales (génétique)</term><term>Protéines virales (métabolisme)</term><term>Régulation de l'expression des gènes viraux</term><term>Temps</term><term>Température</term><term>Vecteurs génétiques</term><term>Virus du SRAS (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Recombinant Proteins</term><term>Viral Matrix Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Escherichia coli</term><term>Protéines de la matrice virale</term><term>Protéines recombinantes</term><term>Protéines virales</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term><term>Recombinant Proteins</term><term>Viral Matrix Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Escherichia coli</term><term>Protéines de la matrice virale</term><term>Protéines recombinantes</term><term>Protéines virales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Viral</term><term>Genes, Viral</term><term>Genetic Vectors</term><term>Genome, Viral</term><term>Open Reading Frames</term><term>Temperature</term><term>Time</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cadres ouverts de lecture</term><term>Gènes viraux</term><term>Génome viral</term><term>Régulation de l'expression des gènes viraux</term><term>Temps</term><term>Température</term><term>Vecteurs génétiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">According to previous studies of SARS-CoV (Severe acute respiratory syndrome coronavirus), a variety of novel accessory genes have been identified in SARS-CoV genome, which were interspersed the structural genes of SARS-CoV and considered to be unique to the SARS-CoV genome. The predicted unknown proteins (PUPs) encoded by the accessory genes might play important roles in the SARS-CoV infection. Three of those genes, called X4, X5 and ORF10, were synthesized and introduced into E. coli to induce expression. SDS-PAGE and Western blotting revealed that the three genes have been expressed in E. coli. The induction of SARS PUPs genes expression in different temperatures, induction times, IPTG concentrations and A values of E. coli cells were performed. The optimal induction condition of SARS-CoV PUPs genes was characterized according to the orthorgonal analysis. The ratio of recombinant proteins of PUPs to total proteins is as follows: X4, 20%; X5, 27.8%; ORF10, 68.5% under the optimum conditions.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Cheng, Ke Di" sort="Cheng, Ke Di" uniqKey="Cheng K" first="Ke-Di" last="Cheng">Ke-Di Cheng</name><name sortKey="Du, Guan Hua" sort="Du, Guan Hua" uniqKey="Du G" first="Guan-Hua" last="Du">Guan-Hua Du</name><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name><name sortKey="Zhu, Ping" sort="Zhu, Ping" uniqKey="Zhu P" first="Ping" last="Zhu">Ping Zhu</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Kong, Jian Qiang" sort="Kong, Jian Qiang" uniqKey="Kong J" first="Jian-Qiang" last="Kong">Jian-Qiang Kong</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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